The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids

► Development of a single cell biosensor for the intracellular detection of amino acids. ► Biosensor-based online monitoring of valine production of a C. glutamicum strain. ► Visualization of cell-to-cell variability of microbial production strains. ► Establishment of a FACS high-throughput screening.