Refolding and purification of recombinant human granulocyte colony-stimulating factor using hydrophobic interaction chromatography at a large scale

► Urea solubilized rhG-CSF was refolded by using HIC. ► Chromatographic conditions have significant effect on refolding of rhG-CSF. ► Protein refolding using liquid chromatography at a protein loading over 1 g.