کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10236439 | 45144 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of two different carriers in a packed bed reactor for endopolygalacturonase production by a yeast strain
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Use of two different carriers in a packed bed reactor for endopolygalacturonase production by a yeast strain Use of two different carriers in a packed bed reactor for endopolygalacturonase production by a yeast strain](/preview/png/10236439.png)
چکیده انگلیسی
A packed bed reactor (PBR) design was tested for the purpose of continuous pectinase production with yeasts, as a possible alternative to the traditional batch process using fungal cultures. Two different carriers - a porous glass (Siran) and a cellulosic carrier obtained from spent grains (barley) - were used to immobilize Kluyveromyces marxianus CCT 3172, a yeast strain secreting endopolygalacturonase. To improve cell distribution throughout the column, part of the outflow was recycled. Cell loads of 0.204 and 0.247 gbiomass/gcarrier were obtained at the top and bottom of the PBR with spent grains, respectively. Using the PBR with Siran as the immobilization support, 0.071 gbiomass/gcarrier was the biomass load at the top of the column while at the bottom a value of 0.147 gbiomass/gcarrier was found. The highest value for pectinase volumetric productivity (PV = 1.68 U/ml h) was achieved in the PBR with Siran for a D = 0.260 hâ1 and a glucose concentration on the inlet of Sin = 40 g/l. Both carriers were suitable for pectinase production. The best results were obtained with a high and uniform biomass concentration in the column, together with high dilution rates and total glucose consumption.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 5, April 2005, Pages 1937-1942
Journal: Process Biochemistry - Volume 40, Issue 5, April 2005, Pages 1937-1942
نویسندگان
Catarina Almeida, TomáÅ¡ Brányik, Pedro Moradas-Ferreira, José Teixeira,