Buffer-mediated activation of Candida antarctica lipase B dissolved in hydroxyl-functionalized ionic liquids

▶ Both the conformation and transesterification activity of CALB solubilized in ILs like [C2OHMIM][BF4] and [C2OHMIM][NO3] are buffer dependent. ▶ CALB possesses a more compact conformation in buffered [C2OHMIM][BF4] or [C2OHMIM][NO3], resulting in the enhancement of transesterification activity with highly stability during recycles. ▶ The knowledge gained in this study may be applicable to homogeneous enzymatic catalysis in organic solvents with hydroxyl group.