Reactivation of immobilized penicillin G acylase: Influence of cosolvents and catalytic modulators

▶ Enzyme average reactivation rate (Rr) strongly depended on reactivation strategy. ▶ Rr was higher in direct incubation in reactivation media than in unfolding/refolding. ▶ Reactivation was complete when adding 100 mM of 7-ADCA with 30% (v/v) of EG. ▶ Non-competitive inhibitors increased global reactivation yield (Yg) and Rr.