کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10254069 | 160905 | 2012 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of stubbing and the double swab method for collecting offender epithelial material from a victim's skin
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
After manual strangulation, epithelial cells originating from the offender can often be found on the skin of the victim. In order to obtain a conclusive DNA profile, it is important to secure as many epithelial cells from the offender and as few epithelial cells from the victim as possible. In this study, two methods for securing offender DNA were compared: the double swab method and an adapted tape-lifting method, so-called stubbing. 50 male volunteers were asked to simulate manual strangulation on the forearm of a female volunteer. After securing the epithelial material, DNA profiles were generated. The contribution of both donors to the samples was determined from the number of detected alleles, specific for each donor, and the average peak height of the donor-specific alleles. For the offender, in all cases except one, partial or full profiles were obtained and no difference between the double swab and the stubbing method was observed. For the victim, fewer alleles were detected by means of double swab than by means of stubbing. In conclusion, the double swab method performs slightly better than the stubbing method. However, from a practical point of view, the stubbing method may be preferred over the double swab technique.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 6, Issue 2, March 2012, Pages 219-223
Journal: Forensic Science International: Genetics - Volume 6, Issue 2, March 2012, Pages 219-223
نویسندگان
Karla G. de Bruin, Saskia M. Verheij, Martine Veenhoven, Titia Sijen,