کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10254082 | 160905 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative real-time PCR (qPCR) assay for human-dog-cat species identification and nuclear DNA quantification
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
We have designed and evaluated a species-specific (human, dog and cat) nuclear DNA identification assay based on the TaqMan® quantitative real-time PCR (qPCR) technology that can simultaneously detect and measure minute quantities of DNA specific to either humans, dogs and/or cats. The fluorogenic triplex assay employs primers and hydrolysis probes that target the human TH01 locus as well as the dog and cat Melanocortin 1 Receptor (MC1R) sequences in a species-specific manner. We also demonstrate that the assay is a highly sensitive, reliable and robust method for identifying and quantifying mixed-species templates of human-dog-cat origin with as little as 0.4Â pg of human and cat nuclear DNA, respectively, and 4.0Â pg of dog nuclear DNA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 6, Issue 2, March 2012, Pages 290-295
Journal: Forensic Science International: Genetics - Volume 6, Issue 2, March 2012, Pages 290-295
نویسندگان
S. Kanthaswamy, A. Premasuthan, J. Ng, J. Satkoski, V. Goyal,