کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10254977 | 161483 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of a gene expression tumour classifier for cancer of unknown primary
ترجمه فارسی عنوان
توسعه و اعتبار طبقه بندی تومور بیان ژن برای سرطان اولیه ناشناخته
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی قانونی
چکیده انگلیسی
Accurate identification of the primary tumour in cancer of unknown primary (CUP) is required for effective treatment selection and improved patient outcomes. The aim of this study was to develop and validate a gene expression tumour classifier and integrate it with histopathology to identify the likely site of origin in CUP. RNA was extracted from 450 formalin fixed, paraffin embedded samples of known origin comprising 18 tumour groups. Whole genome expression analysis was performed using a bead-based array. Classification of the tumours made use of a binary support vector machine, together with recursive feature elimination. A hierarchical tumour classifier was developed and incorporated with conventional histopathology to identify the origins of metastatic tumours. The classifier demonstrated an accuracy of 88% for correctly predicting the tumour type on a validation set of known tumours (n = 94). For CUP samples (n = 49) having a final clinical diagnosis, the classifier improved the accuracy of histology alone for both single and multiple predictions. Furthermore, where histology alone could not suggest any specific diagnosis, the classifier was able to correctly predict the primary site of origin. We demonstrate the integration of gene expression profiling with conventional histopathology to aid the investigation of CUP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pathology - Volume 47, Issue 1, January 2015, Pages 7-12
Journal: Pathology - Volume 47, Issue 1, January 2015, Pages 7-12
نویسندگان
Richard W. Tothill, Fan Shi, Lisa Paiman, Justin Bedo, Adam Kowalczyk, Linda Mileshkin, Evangeline Buela, Robert Klupacs, David Bowtell, Keith Byron,