Detection of ethanol in food: A new biosensor based on bacteria

► A microbial biosensor for determination of ethanol has been developed. ► The sensor comprises bacterial-immobilized eggshell membrane and an O2 electrode. ► The biosensor responds linearly to ethanol in the range 0.050-7.5 mM. ► The detection limit of 0.025 mM (S/N = 3) and the response time is 100 s.