کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10349979 | 863752 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Off-lattice simulation of the solid phase DNA amplification
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Recent simulations of the solid phase DNA amplification (SPA) by J.-F. Mercier et al. [Biophys. J. 85 (2003) 2075] are generalized to include two kinds of primers and the off-lattice character of the primer distribution on the surface. The sigmoidal character of the primer occupation by DNA, observed experimentally, is reproduced in the simulation. We discuss an influence of two parameters on the efficiency of the amplification process: the initial density p0 of the occupied primers from the interfacial amplification and the ratio r of the molecule length to the average distance between primers. The number of cycles required for a complete saturation decreases with p0 roughly as p0â0.26. For r=1.5, the number of occupied primers is reduced by a factor two, when compared to the case of longer molecules. Below r=1.4, the effectivity of SPA is reduced by a factor 100.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Computer Physics Communications - Volume 170, Issue 2, 1 August 2005, Pages 131-136
Journal: Computer Physics Communications - Volume 170, Issue 2, 1 August 2005, Pages 131-136
نویسندگان
M.J. Krawczyk, K. KuÅakowski,