کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
103751 161404 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High-resolution melting analysis for genotyping Duffy, Kidd and Diego blood group antigens
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
High-resolution melting analysis for genotyping Duffy, Kidd and Diego blood group antigens
چکیده انگلیسی

High-resolution melting (HRM) analysis is a simpler genotyping method than allele-specific PCR, PCR-restriction fragment length polymorphism and multiplex PCR. Duffy, Kidd and Diego are clinically important blood group antigens. We used a novel method to genotype these three blood group antigens.Purified genomic DNA extracts of blood samples (354 Duffy, 347 Kidd and 457 Diego) were amplified using specific amplification primers. HRM curves were obtained by HRM analysis.Results were in complete concordance with those obtained for previous phenotypes and genotypes. Nucleotide substitutions for these blood group antigens were differentiated by the HRM curves. HRM analysis is a simple genotyping method and is an alternative to serological typing.Our results suggest that HRM analysis can also be used for genotyping blood group antigens whose allotype specificity is determined by single nucleotide substitutions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Legal Medicine - Volume 13, Issue 1, January 2011, Pages 1–6
نویسندگان
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