Structural changes of lignocelluloses by a nonionic surfactant, Tween 20, and their effects on cellulase adsorption and saccharification

► Treatment of lignocelluloses with Tween 20 contributed to the formation of 10- to 50-nm pores. ► Tween 20 treatment increased cellulase adsorption rate and amount onto lignocelluloses substantially. ► Structural effect of Tween 20 depended on lignin contents and crystallinity.