Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp.

► Non-denaturing isolation process yielding algae soluble protein isolate (ASPI). ► Successful removal of intense color during algae protein isolation. ► Nitrogen-to-protein conversion factors for quantitative description of ASPI recovery. ► Physico-chemical characterization of ASPI. ► ASPI is completely soluble at pH 5.5-8.0 at all ionic strengths tested.