Optimization of CO2 laser-based pretreatment of corn stover using response surface methodology

► We found CO2 laser could effectively pretreat corn stover. ► We demonstrate that CO2 laser has potential to enhance the bioconversion. ► Corn stover was analyzed with SEM, and evaluated effect of pretreatments. ► CO2 laser pretreatment could cause structure breakage of the corn stover material. ► CO2 laser pretreatment reduced the hydrolysis time.