Industrial yeast strain engineered to ferment ethanol from lignocellulosic biomass

► Industrial strain of Saccharomyces cerevisiae engineered to ferment pretreated lignocellulosic material. ► Strains produce ethanol from pretreated biomass without the addition of exogenously produced enzymes. ► Highest performing engineered yeasts secreting cellulolytic enzymes selected based on cellulase activity assays. ► Ethanol fermentation from pretreated corn stover with 63% conversion in 96 h. ► First example of Simultaneous Saccharification and Fermentation (SSF) from biomass without exogenously produced enzymes.