کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10395486 | 888979 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhancement of riboflavin production with Bacillus subtilis by expression and site-directed mutagenesis of zwf and gnd gene from Corynebacterium glutamicum
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کلمات کلیدی
CDWRU5PPRPPd-Glyceraldehyde 3-phosphateL-PheDPDPEPFBPTCAPPPL-phenylalanineflavin adenine dinucleotideFMNG6PD6-phosphogluconate dehydrogenase - 6-فسفوگلاوکونات دهیدروژنازLC–MS/MS - LC-MS / MStricarboxylic acid - اسید تری کربوکسیلیکFAD - بدdihydropyrimidine dehydrogenase - دی هیدروپیریمیدین دهیدروژنازRiboflavin - ریبوفلاوین، ویتامین B2fructose 1,6-bisphosphate - فروکتوز 1،6-بیسفسفاتphosphoribosyl pyrophosphate - فسفریبوسیل پیرو فسفاتphosphoenolpyruvate - فسفوآنولپیرواتflavin mononucleotide - فلاون مونونوکلئوتیدLuria–Bertani - لواریا بارتانیminimal medium - متوسط متوسطpentose phosphate pathway - مسیر پنتوز فسفاتcell dry weight - وزن خشک سلولیglucose-6-phosphate dehydrogenase - گلوکز 6-فسفات دهیدروژناز
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Enhancement of riboflavin production with Bacillus subtilis by expression and site-directed mutagenesis of zwf and gnd gene from Corynebacterium glutamicum Enhancement of riboflavin production with Bacillus subtilis by expression and site-directed mutagenesis of zwf and gnd gene from Corynebacterium glutamicum](/preview/png/10395486.png)
چکیده انگلیسی
Zwf (code for glucose-6-phosphate dehydrogenase) and gnd (code for 6-phosphogluconate dehydrogenase) genes from Corynebacterium glutamicum were firstly cloned, and then site-directed mutagenesis was successfully introduced to remove allosteric inhibition by intracellular metabolites. Expression of the mutant zwf and gnd in Bacillus subtilis RH33 resulted in significant enhancement of riboflavin productivity, while the specific growth rate decreased slightly and the specific glucose uptake rate was unchanged. Introduction of the mutant zwf and gnd led to approximately 18% and 22% increased riboflavin production, respectively. An improvement by 31% and 39% of the riboflavin production was obtained by co-expression of the mutated dehydrogenases in shaker flask and fed-batch cultivation. Intracellular metabolites analysis indicated that metabolites detected in pentose phosphate pathway or riboflavin synthesis pathway of engineered strains showed higher concentration, while TCA cycle and glycolysis metabolites detected were lower abundance than that of parent strain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 102, Issue 4, February 2011, Pages 3934-3940
Journal: Bioresource Technology - Volume 102, Issue 4, February 2011, Pages 3934-3940
نویسندگان
Zhiwen Wang, Tao Chen, Xianghui Ma, Zhuo Shen, Xueming Zhao,