Immobilization of enzyme on long period grating fibers for sensitive glucose detection

► Glucose oxidase (GOD) has been covalently immobilized on the Long Period Grating (LPG) fiber surface to construct a sensitive glucose sensor. ► Detailed investigations by FTIR spectrometry, Raman spectrometry, FESEM and confocal laser scanning microscopy have confirmed the successful enzyme immobilization. ► Constructed GOD immobilized LPG sensor is responsive to physiologically important glucose concentrations. Various important sensor optimizations parameters have been established.