Highly sensitive detection of Salmonella typhi using surface aminated polycarbonate membrane enhanced-ELISA

► Neat and clean chemical derivatization of polycarbonate membrane. ► Sandwich immunoassay was developed on mPC. ► Detection of whole cell S. typhi bacteria was done without any pre-enrichment. ► Sensitivity of 2000 cells/ml was achieved which is below infectious dose. ► Covalent binding of Abs amplified signal and abated leaching. ► The assay was found to be better than qualitative kits available in markets.