A non-invasive method for an in vivo assessment of corneal epithelium permeability through tetrapolar impedance measurements

► Impedance measurements were performed with four electrodes placed on the corneal surface. ► Methods based on the electrical properties are the most suitable to evaluate corneal permeability. ► The permeability of a rabbit corneal epithelium was monitored. ► The sensor can be used for in vivo studies and clinical settings.