کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10430103 | 909879 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring](/preview/png/10430103.png)
چکیده انگلیسی
Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H2O2-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency, Imax, KMapp, detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is explained by a high stability of this enzyme in presence of H2O2. The higher storage and operational stability of RPTP-based biosensor as well as its capability to measure hydrogen peroxide under acidic conditions connect with an extremely high thermal and pH-stability of RPTP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 21, Issue 5, 15 November 2005, Pages 742-748
Journal: Biosensors and Bioelectronics - Volume 21, Issue 5, 15 November 2005, Pages 742-748
نویسندگان
Inna S. Alpeeva, Mihaela Niculescu-Nistor, Jaime Castillo Leon, Elisabeth Csöregi, Ivan Yu. Sakharov,