کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10529009 | 960770 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous determination of two active ingredients in Flos daturae by capillary electrophoresis with electrochemiluminescence detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The coupling of Ru(bpy)32+ based electrochemiluminescence (ECL) detection with capillary electrophoresis (CE) was developed for the simultaneous determination of the two major active ingredients (atropine and scopolamine) in Flos daturae. Parameters related to the separation and detection were discussed and optimized. It was proved that 20Â mM phosphate buffer at pH 8.48 could achieve the most favorable resolution, and the high sensitivity of detection was obtained by maintaining the detection potential at 1.2Â V. Under the optimized conditions: ECL detection at 1.2Â V, 20Â mM phosphate buffer at pH 8.48, 5Â mM Ru(bpy)32+ and 50Â mM phosphate buffer at pH 7.48 in the detection reservoir, detection limits of 5Â ÃÂ 10â8Â mol/l for atropine and 1Â ÃÂ 10â6Â mol/l for scopolamine were obtained. Relative standard derivations of the ECL intensity and the migration time were 5.16 and 0.71% for atropine and 5.07 and 1.22% for scopolamine, respectively. Developed method was successfully applied to determine the amounts of both alkaloids in Flos daturae. A baseline separation for atropine and scopolamine was achieved within 11Â min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 545, Issue 2, 27 July 2005, Pages 137-141
Journal: Analytica Chimica Acta - Volume 545, Issue 2, 27 July 2005, Pages 137-141
نویسندگان
Ying Gao, Yiling Tian, Erkang Wang,