کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10529015 | 960770 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous determination of total homocysteine, cysteine and methionine in hypothyroid patients' plasma by liquid chromatography using platinum/poly(methyl violet) modified electrode
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The fabrication and application of a novel electrochemical detection (ED) system with the platinum/poly(methyl violet) (Pt/MV) chemically modified electrode (CME) for high performance liquid chromatography (HPLC) were described. The Pt particles deposited on the poly-MV film were characterized by atomic force microscope (AFM). It was found that the Pt/MV CME exhibited efficiently electrocatalytic effect on the current responses of cysteine (Cys), homocysteine (Hcy) and methionine (Met) with relatively high sensitivity, stability and long-life of activity. In HPLC-ED, these three amino acids had good and stable current responses at the CME and their linear ranges were over three orders of magnitude (R â¥Â 0.9996) with the detection limits being 7.5 Ã 10â8 mol Lâ1 for Cys, 1.0 Ã 10â7 mol Lâ1 for Hcy, 5.0 Ã 10â7 mol Lâ1 for Met. The application of this method coupled with microdialysis sampling for the determination of Cys, Hcy and Met in plasma from patients with hypothyroidism was satisfactory.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 545, Issue 2, 27 July 2005, Pages 182-188
Journal: Analytica Chimica Acta - Volume 545, Issue 2, 27 July 2005, Pages 182-188
نویسندگان
Haihong Xu, Wen Zhang, Wei Zhu, Dan Wang, Jun Ye, Katsunobu Yamamoto, Litong Jin,