کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10529474 960807 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measurement of diffusion and partition coefficients of ferrocyanide in protein-immobilized membranes
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Measurement of diffusion and partition coefficients of ferrocyanide in protein-immobilized membranes
چکیده انگلیسی
True diffusion (Dm) and partition (α) coefficients for the transport of potassium ferrocyanide through diaphorase (Dp)- and bovine serum albumin (BSA)-glutaraldehyde (GA) membranes with different cross-linking degrees of 1-8% GA concentrations immobilized on gold electrodes are investigated by using potential-step method and rotating-disk-electrode method. The thickness of dry and hydrated immobilized membranes is accurately measured by the focus-difference method with a reflection microscope. The thickness of hydrated Dp-GA and BSA-GA membranes are about 1.4 and 2.4 times that of dry membranes, respectively. In addition, the actual area of electrode surface is calculated by the charge amount of chemisorbed oxygen on gold electrode. Owing to the increase of swelling degree and net negative charge of the immobilized membranes, the values of Dm and α for both of Dp-GA and BSA-GA membranes enlarge and decrease with increase of GA concentration, respectively. Furthermore, BSA-GA membranes possess greater Dm and α than those of Dp-GA membranes due to the thinner thickness and the greater swelling degree of BSA-GA membranes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 532, Issue 2, 14 March 2005, Pages 209-214
نویسندگان
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