کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10529692 | 960816 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
1H NMR-based metabonomics for the diagnosis of inborn errors of metabolism in urine
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
1H NMR-based metabonomics was used for the detection and diagnosis of inborn errors of metabolism from urine samples. 1D 1H NMR spectra from 47 normal, 9 phenylketonuric (PKU) newborns and 1 maple syrup urine disease (MSUD) child were obtained and investigated. Urine 1H NMR spectra of normal, PKU and MSUD samples exhibited differences concerning the phenylalanine (Phe) and branched-chain amino acids (leucine, valine, isoleucine) resonances, respectively. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were applied in order to establish adequate models for discrimination between pathological and normal samples. Normalization of the spectra was based to the total spectral intensity or to creatinine peak. Different data transformation procedures were used. Discrimination of PKU and MSUD samples from normal samples was achieved by the different models produced by PCA and PLS-DA. Comparing the two methods of statistical analysis, PLS-DA was found to lead to a most proper discrimination when all pathological samples were used, while PCA proved suitable to identify every single pathological sample among the physiological ones. Thus, 1H NMR in urine can be considered as an alternative to blood spots in order to develop a mass-screening method, which does not require sample pre-treatment and avoids any painful procedure for the newborns.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 542, Issue 2, 29 June 2005, Pages 169-177
Journal: Analytica Chimica Acta - Volume 542, Issue 2, 29 June 2005, Pages 169-177
نویسندگان
Maria A. Constantinou, Evangelos Papakonstantinou, Manfred Spraul, Sophia Sevastiadou, Christos Costalos, Michael A. Koupparis, Kleopatra Shulpis, Anna Tsantili-Kakoulidou, Emmanuel Mikros,