Advances in ion mobility spectrometry-mass spectrometry reveal key insights into amyloid assembly

► IMS-MS provides insights into the mechanism of amyloid formation. ► IMS-MS enables individual conformers of unfolded proteins to be characterized. ► IMS-MS separates and characterizes individual oligomers in a heterogeneous ensemble. ► Ligand binding to specific protein conformers can be detected using IMS-MS.