Introduction of a thermophile-sourced ion pair network in the fourth beta/alpha unit of a psychophile-derived triosephosphate isomerase from Methanococcoides burtonii significantly increases its kinetic thermal stability

► Novel TIM from Methanococcoides burtonii. ► Redesign of a psychrophile TIM to increase its thermal (kinetic) stability. ► Import (imitation) of key ion pair network from P. furiosus TIM. ► Marked effect of increasing sub-domain stability on protein kinetic stability. ► Effect of reducing global cooperativity of unfolding upon rate of unfolding.