Design and characteristics of a stable protein scaffold for specific binding based on variable lymphocyte receptor sequences

► Design of novel variable lymphocyte receptor protein (dVLR). ► dVLR remains monomeric in various protein concentration. ► dVLR shows high thermostability over broad pH range. ► The highest stability was observed at pH 6.0 with denaturation temperature 73.9 °C. ► dVLR is suitable for biotechnological applications.