Keywords: Excluded volume effect; Osmolytes; Monosaccharides; Oligosaccharides; Degree of stabilization; Apo; α-lactalbumin; α-LA; Ribonuclease A; RNase; Guanidinium chloride; GdmCl; Glucose; Glc; Galactose; Gal; Fructose, Fru;
مقالات ISI (ترجمه نشده)
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Keywords: PEG; polyethylene glycol; FKBP; FK-506 binding protein; HSA; human serum albumin; GdmCl; guanidinium chloride; Tm; midpoint of denaturation; ÎHm; enthalpy change at Tm; Trp; tryptophan; bOBP; bovine odorant binding protein; BSA; bovine serum albumin; ANS
Keywords: AcLDL; acetyl LDL; apo; apolipoprotein; BSA; bovine serum albumin; CVD; cardiovascular disease; CE; cholesteryl ester; CERM; cholesteryl ester-rich microemulsion; CETP; cholesteryl ester transfer protein; FC; free cholesterol; GdmCl; guanidinium chloride;
Glycine-rich loop encompassing active site at interface of hexameric M. tuberculosis Eis protein contributes to its structural stability and activity
Keywords: wtRvEis; wild-type RvEis; Eis; enhanced intracellular survival; GdmCl; guanidinium chloride; Tm; melting temperature; Cm; melting concentration; KAN; kanamycin; SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel electrophoresis; ANS; 8-anilino-1-naphthal
The histidine phosphocarrier protein, HPr, binds to the highly thermostable regulator of sigma D protein, Rsd, and its isolated helical fragments
Keywords: Binding; Circular dichroism; Fluorescence; Histidine-phosphocarrier-protein; Isothermal titration calorimetry; NMR; Thermostability; ANS; 8-naphthalene-1-sulfonic acid; ASA; accessible surface area; BLI; biolayer interferometry; CD; circular dichroism; DL
NADP+ binding effects tryptophan accessibility, folding and stability of recombinant B. malayi G6PD
Keywords: ANS; 1-anilinonaphthalene-8-sulfonate; BmG6PD; Brugia malayi glucose-6-phosphate dehydrogenase; G6P; glucose 6-phosphate; GdmCl; guanidine hydrochloride; KI; potassium iodide; Brugia malayi G6PD; Dissociation constant; Homology model;
Multiple disulfide bridges modulate conformational stability and flexibility in hyperthermophilic archaeal purine nucleoside phosphorylase
Keywords: SsMTAPII; 5â²-deoxy-5â²-methylthioadenosine phosphorylase II from Sulfolobus solfataricus; PNP; purine nucleoside phosphorylase; AdoMet; S-adenosylmethionine; MTA; 5â²-deoxy-5â²-methylthioadenosine; PDI; protein-disulfide isomerase, DSC, differential
The E. coli thioredoxin folding mechanism: The key role of the C-terminal helix
Keywords: CD; circular dichroism; CTH; C-terminal α-helix; DLS; dynamic light scattering; ESI-MS; electrospray ionization mass spectrometry; GdmCl; guanidinium chloride; HB; hydrogen bond; Iâ and Iâ-sim; high energy intermediate state inferred from experiments
Structural diversity of a collagen-binding matrix protein from the byssus of blue mussels upon refolding
Keywords: CD; circular dichroism; ESI; electrospray ionization; FTIR; Fourier transform infrared spectroscopy; GdmCl; guanidinium hydrochloride; IAEDANS; 5-[2-[(2-Iodo-1-oxoethyl)amino]ethylamino]-1-naphthalenesulfonic acid; LC; liquid chromatography; MALDI; matrix
Rv3868 (EccA1), an essential component of the Mycobacterium tuberculosis ESX-1 secretion system, is thermostable
Keywords: NT-Rv3868; N-terminal domain of Rv3868; CT-Rv3868; C-terminal ATP-binding domain of Rv3868; CD; circular dichroism; GdmCl; guanidine hydrochloride; ESX-1 secretion system; AAA+ ATPase; Thermostability; Charged and aliphatic amino acids;
Enhancing the biophysical properties of mRFP1 through incorporation of fluoroproline
Keywords: NCAA; non-canonical amino acid incorporation; FP; fluoroproline; (4R)-FP; (2S, 4R)-4-fluoroproline; (4S)-FP; (2S, 4S)-4-fluoroproline; MM; minimal media; GdmCl; guanidine hydrochloride; mRFP1; mRFP1-P63A; (2S, 4R)-4-fluoroproline; Non-canonical amino acid
Variant of the Thermomyces lanuginosus lipase with improved kinetic stability: A candidate for enzyme replacement therapy
Keywords: cmc; Critical micelle concentration; DecM; Decyl maltoside; DSC; Differential scanning calorimetry; GC; Sodium glycocholate hydrate; GdmCl; Guanidinium chloride; ITC; Isothermal titration calorimetry; SDS; Sodium dodecyl sulfate; SDS-PAGE; Sodium dodecyl
Temperature adaptations in psychrophilic, mesophilic and thermophilic chloride-dependent alpha-amylases
Keywords: Alpha-amylase; Extremophiles; Antarctic; Differential scanning calorimetry; Protein stability; AHA; α-amylase from Pseudoalteromonas haloplanktis (psychrophile); DMA; α-amylase from Drosophila melanogaster (ectothermic mesophile); PPA; α-amylase from p
Role of disulfide bonds in conformational stability and folding of 5â²-deoxy-5â²-methylthioadenosine phosphorylase II from the hyperthermophilic archaeon Sulfolobus solfataricus
Keywords: MTAP; 5â²-deoxy-5â²-methylthioadenosine phosphorylase; SsMTAPII; 5â²-deoxy-5â²-methylthioadenosine phosphorylase II from Sulfolobus solfataricus; PNP; purine nucleoside phosphorylase; MTA; 5â²-deoxy-5â²-methylthioadenosine; SDS-PAGE; sodium dodecyl
Design and characteristics of a stable protein scaffold for specific binding based on variable lymphocyte receptor sequences
Keywords: VLR; variable lymphocyte receptor; VLRB; variable lymphocyte receptor type B; dVLR; designed VLR; LRR; leucine-rich repeat; LRRNT; leucine-rich repeat N-terminal capping region; LRR1; the first leucine-rich repeat; LRRV; leucine-rich repeat variable; LRRV
Purification and characterization of Plasmodium yoelii adenosine deaminase
Keywords: EHNA; erythro-9-(2-hydroxy-3-nonyl) adenine; pCMB; p-chloromercuribenzoate; NEM; N-ethylmaleimide; EDTA; ethylenediamine tetraacetic acid; GdmCl; guanidinium hydrochloride; DEPC; di-ethyl pyrocarbonate; Adenosine deaminase; Drug resistance; Inhibitor; Mal
Folding studies of purified LamB protein, the maltoporin from the Escherichia coli outer membrane: Trimer dissociation can be separated from unfolding
Keywords: octylPOE; n-octyl (polydisperse)oligo-oxyethylene; SDS; sodium dodecyl sulfate; DM; dodecylmaltoside; GdmCl; guanidinium chloride; DTT; dithiothreitol; Hepes; N-2-Hydroxypiperazine-N-ethanesulfonic acid; CD; circular dichroism; PAGE; polyacrylamide gel el
Enhanced prion protein stability coupled to DNA recognition and milieu acidification
Keywords: PrP; prion protein; PrPSc; prion scrapie; mrPrP; recombinant murine prion protein; PrPC; cellular prion protein; TSEs; transmissible spongiform encephalopathies; GdmCl; guanidinium chloride; NA; nucleic acid.; Prion; DNA; Stability; Unfolding; pH; Urea; G
Hsp70-1 from Plasmodium falciparum: Protein stability, domain analysis and chaperone activity
Keywords: CD; circular dichroism; GdmCl; guanidium chloride; G6PDH; Glucose-6-phosphate dehydrogenase; NBD; nucleotide-binding domain; P-Ctr; substrate-binding domain with C-terminal; Pfhsp70-1; Plasmodium falciparum heat shock protein 70-1; SBD; substrate-binding
Metal-triggered changes in the stability and secondary structure of a tetrameric dihydropyrimidinase: A biophysical characterization
Keywords: ANS; 8-anilinonaphtalene-1-sulfonic acid; ASA; accessible solvent area; DSC; differential scanning calorimetry; GdmCl; guanidine hydrochloride; [GdmCl]1/2; the denaturation midpoint of the chemical-denaturation; FTIR; Fourier transform infrared spectrosco
Conformational stability and integrity of α-amylase from mung beans: Evidence of kinetic intermediate in GdmCl-induced unfolding
Keywords: VrAMY; Vigna radiata α-amylase; AMY 1; barley α-amylase 1; AMY 2; barley α-amylase 2; BAA; Bacillus amyloliquefaciens α-amylase; BLA; Bacillus licheniformis α-amylase; PPA; porcine pancreatic amylase; GdmCl; Guanidine hydrochloride; DTT; Dithiothreit
Sequence and stability of the goat cytochrome c
Keywords: ÎGDo; Gibbs energy change in the absence of denaturant; ÎGs; solvation energy of chain folding; GdmCl; guanidinium chloride; Folding and stability; Molecular modeling; Goat cytochrome c; Bovine cytochrome c; Circular dichroism;
A new method for determining the constant-pressure heat capacity change associated with the protein denaturation induced by guanidinium chloride (or urea)
Keywords: CD; circular dichroism; [θ]λ; mean residue ellipticity at wavelength, λ nm; RNase-A; ribonuclease-A; apo-La; apo-α-lactalbumin; ctg; chymotrypsinogen A; GdmCl; guanidinium chloride.; Protein folding; Protein denaturation; Heat capacity;
Guanidinium chloride and urea denaturations of β-Lactoglobulin A at pH 2.0 and 25 °C: The equilibrium intermediate contains non-native structures (helix, tryptophan and hydrophobic patches)
Keywords: β-lgA; β-lactoglobulin A; ε; molar absorption coefficient; Îε292; difference molar absorbance at 292 nm; [θ]222; mean residue ellipticity at 222 nm; ANS; 8-anilino-1-naphthalene sulphonic acid; GdmCl; guanidinium chloride; TFE; trifluoroethanol; y
Selecting for antibody scFv fragments with improved stability using phage display with denaturation under reducing conditions
Keywords: scFv; single-chain Fv antibody fragment; VH; heavy chain of an antibody Fv fragment; VL; light chain of an antibody Fv fragment; GdmCl; guanidinium chloride; TSH; thyroid-stimulating hormone; DTT; dithiotreitol; GSH; reduced glutathione; GSSG; oxidized gl