کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5371726 1503966 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced prion protein stability coupled to DNA recognition and milieu acidification
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Enhanced prion protein stability coupled to DNA recognition and milieu acidification
چکیده انگلیسی

The prion protein (PrP) is the major agent involved in the transmissible spongiform encephalopathies (TSEs). Nucleic acids have been reported to bind PrP with high affinity, although the physiopathological roles for recognition are still not clear. In this work we investigate the stability of a soluble, 1:1 complex formed between an 18 base-pair DNA fragment and the full-length murine recombinant prion protein (mrPrP). DNA confers a gain in mrPrP stability against urea and guanidinium denaturation, which is enhanced at lower pHs and in moderate concentrations of NaCl. We discuss the cooperative folding transition coupled to DNA binding and acidification in terms of the possible cellular scenarios found during complex internalization and degradation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 141, Issues 2–3, May 2009, Pages 135-139
نویسندگان
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