Effect of NaCl on the conformational stability of the thermophilic γ-glutamyltranspeptidase from Geobacillus thermodenitrificans: Implication for globular protein halotolerance

► GthGT is active in the presence of 4 M NaCl. ► GthGT is stabilized by the presence of NaCl. ► NaCl stabilizing effect has been studied by a statistical thermodynamic model. ► Negative charged residues on the surface are important for protein halotolerance.