Influence of lipid composition on physicochemical properties of nanoliposomes encapsulating natural dipeptide antioxidant l-carnosine

► In this study, we prepared nanoliposomes from different lipids (DOPC, POPC and DPPC) by thin film hydration method. ► The encapsulation efficacy was determined by using a precise approach 1H NMR without the need for physical separation. ► The morphology of nanoliposomes on mica surface was also investigated using Atomic Force Microscopy. ► By operating in soft tapping mode, liposome diameters remain comparable to the heights of the vesicles. ► The elasticity/viscosity and hydrophilicity/hydrophobicity properties of liposomes surfaces influenced the phase images.