Validation of a UHPLC-FLD method for the simultaneous quantification of aflatoxins, ochratoxin A and zearalenone in barley

► Validated UHPLC-FLD determination of aflatoxins, ochratoxin A and zearalenone in barley. ► Simultaneous acetonitrile/water extraction and IAC purification. ► Aflatoxins detection improved with photochemical reaction. ► Most mycotoxins values found in 20 samples were