کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10547841 965301 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A modified supported bilayer/diblock polymer - Working towards a tunable coating for capillary electrophoresis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A modified supported bilayer/diblock polymer - Working towards a tunable coating for capillary electrophoresis
چکیده انگلیسی
A surfactant bilayer/diblock polymer coating was previously developed for the separation of proteins. The coating consisted of a mixture of the cationic surfactant dioctadecyldimethylammonium bromide (DODAB) and the neutral polymer poly-oxyethylene (POE) 40 stearate (Journal of Chromatography A 1130 (2006) 265-271). Herein an improved method of generating DODAB/POE stearate coatings is demonstrated, which yields more predictable EOF, more stable coatings, greater average efficiencies and easier method development. In this sequential preparation method the DODAB is first flowed through the capillary, followed by a flow of the POE stearate (sequential method). A tunable EOF (−2.40 to −0.17 × 10−4 cm2/Vs) is achieved by varying the POE chain length (8, 40 and 100 oxyethylene units). Mixtures of POE 8 and POE 40 stearate enabled continuous variation in EOF from −2.44 to −0.42 × 10−4 cm2/Vs. Separations of basic proteins yielded efficiencies of 760 000-940 000 plates/m. Coatings formed using the sequential method were more stable over a larger number of runs (%RSD for migration times: 0.7-1.0% over 30 runs) than those formed using the original mixed method (%RSD: 2.4-4.6% over 14 runs). The ability to tune the EOF is important in maximizing the resolution of analytes with similar electrophoretic mobilities. Histone proteins are separated on a sequentially coated capillary with resolution of nine possible subtypes. Acidic proteins are separated on a sequentially coated capillary at pH 6.4.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 1, 7 January 2011, Pages 178-184
نویسندگان
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