کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10547937 965401 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetic study of cytochrome P450 3A4 activity on warfarin by capillary electrophoresis with fluorescence detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Kinetic study of cytochrome P450 3A4 activity on warfarin by capillary electrophoresis with fluorescence detection
چکیده انگلیسی
The use of capillary electrophoresis (CE) for the determination of cytochrome P450 3A4 (CYP3A4) activity with R-warfarin as a substrate was investigated. CYP3A4 activity was determined by the quantitation of the product, 10-hydroxywarfarin, based on separation by CE. The separation conditions were as follows: capillary, 80.5 cm (75 μm i.d., 60 cm effective length); 50 mM sodium phosphate buffer (pH 6.5); 23 kV (90 μA) applied voltage; fluorescence detection, excitation wavelength, 310 nm, emission wavelength, 418 nm; capillary temperature, 37 °C. With the developed CYP3A4 activity assay and the Lineweaver-Burk equation, the Michaelis-Menten parameters Km and Vmax for formation of 10-hydroxywarfarin from R-warfarin in the presence of CYP3A4 were calculated to be 166 ± 12 μM and 713 ± 14 pmol/min/nmol (or 91.4 pmol/min/mg) CYP3A4, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1082, Issue 2, 5 August 2005, Pages 235-239
نویسندگان
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