کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10547975 | 965404 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and optimization of a method for the analysis of Brazilein by HPLC with electrochemical detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The aim of this study was to establish an easy and accurate method for the determination of Brazilein in plant samples due to its potential pharmacological activities. High-performance liquid chromatography (HPLC) with electrochemical detection (ED) was used for the assay of Brazilein in this study for the first time. Crucial influence parameters including concentration of dodecane-1-sulfonic acid sodium salt (DSASS), inorganic modifier, tetrabutyl-ammonium hydroxide solution (TBAOH), and applied potential of proposed method were investigated. The proposed method is simple, rapid (analysis time: â¼10 min), sensitive [(detection limit: 0.6 ng per injection (20 μl) at a signal-noise ratio 3:1)], highly selective and precise (intra- and inter-day precisions were within 5%, n = 7). The calibration graph of Brazilein was linear in the range 0.6-150 ng per injection 20 μl. Recovery of Brazilein was over 92% by standard addition method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1077, Issue 1, 3 June 2005, Pages 44-48
Journal: Journal of Chromatography A - Volume 1077, Issue 1, 3 June 2005, Pages 44-48
نویسندگان
Xiaoling Yan, Wei Wang, Dongming Xing, Yunan Zhao, Lijun Du,