کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10548002 | 965408 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of ethyl glucuronide in human serum by capillary electrophoresis with sample self-stacking and indirect detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Analysis of ethyl glucuronide in human serum by capillary electrophoresis with sample self-stacking and indirect detection Analysis of ethyl glucuronide in human serum by capillary electrophoresis with sample self-stacking and indirect detection](/preview/png/10548002.png)
چکیده انگلیسی
Ethyl glucuronide (EtG), a metabolite of ethanol, is a marker of recent alcohol consumption. In the past few years, its analysis in body fluids has attracted considerable attention because it closes a gap between short time and long time alcohol markers such as ethanol and carbohydrate-deficient transferrin, respectively. The capillary zone electrophoresis (CZE) analysis of EtG in model mixtures and human serum is reported using uncoated and coated fused-silica capillaries together with acidic buffers in the pH range between 3.2 and 4.4 and indirect detection. In these approaches, separation of EtG from endogenous macro- and microcomponents (anionic serum components of high and low concentration, respectively) is based upon transient isotachophoretic stacking referred to as sample self-stacking. The selection of a favorable buffer co-ion and pH is shown to be crucial for optimized sensitivity. A buffer composed of 10 mM nicotinic acid and É-aminocaproic acid (pH 4.3) is demonstrated to provide a detection limit for EtG in serum of 0.1 μg/ml, a value that is relevant for clinical and forensic purposes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1081, Issue 1, 15 July 2005, Pages 2-8
Journal: Journal of Chromatography A - Volume 1081, Issue 1, 15 July 2005, Pages 2-8
نویسندگان
L. KÅivánková, J. Caslavska, H. MaláÅ¡ková, P. Gebauer, W. Thormann,