کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10548038 965412 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Autoanalyzer for continuous fractionation and quantitation of the polyphenols content in wines
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Autoanalyzer for continuous fractionation and quantitation of the polyphenols content in wines
چکیده انگلیسی
A simple continuous flow autoanalyzer for the on-line fractionation of the polyphenols content in wines is proposed. The target compounds are isolated from the matrix by solid-phase extraction on an RP-C18 sorbent column, using selective solvents for the sequential elution of each polyphenol family. Moreover, evaporative light scattering detection (ELSD) is used for the first time for the on-line monitorization of the three polyphenol fractions present in the wine samples. Thus, a single sample injection is required to determine the global concentration of the three selected polyphenol fractions and the whole analysis is completed within a few minutes. Three calibration graphs were constructed for quantitative analysis of the global compounds concentration in every fraction and covered the range 5-300 mg l−1 (expressed as gallic acid). Average repeatability, expressed as relative standard deviation, was 4%. The proposed autonalyzer was applied to the analysis of a variety of commercial wine samples. The results obtained were compared with those provided by the Folin-Ciocalteau method, being similar in all instances.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1081, Issue 2, 22 July 2005, Pages 127-131
نویسندگان
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