کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10548071 | 965413 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lysozyme purification from tobacco extract by polyelectrolyte precipitation
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کلمات کلیدی
CMCPAABCADHBisoelectricBSA - BSAbovine serum albumin - آلبومین سرم گاوPrecipitation - بارندگیbicinchoninic acid - بیسینکنینیک اسیدTransgenic tobacco - تنباکوی ترانس ژنDihydroxybenzenes - دی هیدروکسیبنزن هاlysozyme - لیزوزیم Recombinant protein - پروتئین های نوترکیبpolyelectrolyte - پلی الکترولیتPoly(acrylic) acid - پلی اکریلیک اسیدCarboxymethyl cellulose - کربوکسی متیل سلولز
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: Lysozyme purification from tobacco extract by polyelectrolyte precipitation Lysozyme purification from tobacco extract by polyelectrolyte precipitation](/preview/png/10548071.png)
چکیده انگلیسی
Tobacco is widely used as a model plant for feasibility studies of recombinant protein production from transgenic plants. However, dealing with large quantities of biomass to recover recombinant proteins is a challenge for down-stream processing. In this study, the effect of isoelectric precipitation on native tobacco protein was first studied. Among the three acids studied, hydrochloric acid is shown to be more effective than acetic or citric acid, and at pH 4, 60% of native tobacco protein was precipitated by HCl. Egg white lysozyme was used as the model protein to test the feasibility of polyelectrolyte precipitation in protein recovery from tobacco extract. Precipitation of lysozyme at pH 7 was shown ineffective probably because of the interference of polyphenolic acids. However, after isoelectric precipitation at pH 5 poly(acrylic) acid (PAA) was shown to precipitate 85% of the soluble lysozyme when the polymer dosage was increased to 1.5Â mg polymer/mg lysozyme, while negligible amounts of native tobacco protein was co-precipitated. Lysozyme precipitation by PAA in tobacco extract obtained at pH 5 was also studied, and lysozyme yield was significant improved.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1069, Issue 1, 25 March 2005, Pages 107-112
Journal: Journal of Chromatography A - Volume 1069, Issue 1, 25 March 2005, Pages 107-112
نویسندگان
Chenming Zhang, Raymond Lillie, Jackie Cotter, David Vaughan,