کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10548155 965427 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Separation of phytosterol oxidation products by combination of different polarity gas chromatography capillary columns
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Separation of phytosterol oxidation products by combination of different polarity gas chromatography capillary columns
چکیده انگلیسی
The number of characterized phytosterol oxidation products (POPs) from both ring- and side-chain structures has increased during recent decades, resulting in difficulties in the separation of POPs on different gas chromatography (GC) capillary columns. The main objective of this study was to separate a mixture of 29 purified and characterized oxidation products from sito-, campe- and stigmasterol using GC capillary columns with different polarity. For the first time in the area of POPs analysis, the separation efficiency of the combination of two capillary GC columns with different polarities was investigated. A non-polar 5% phenyl coated (DB5-MS) and a mid-polar 35% phenyl coated (DB35-MS) column was combined with a pressfit connector. The main improvement was enhanced base line separation for many of the analyzed POPs, compared with the separations achieved using the individual columns. However, three pairs of POPs co-eluted: 24-hydroxysitosterol/campesterol-5β,6β-epoxide, stigmasterol-5β,6β-epoxide/campesterol-5α,6α-epoxide and stigmasterol-5α,6α-epoxide/campestanetriol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1064, Issue 2, 4 February 2005, Pages 213-217
نویسندگان
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