کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10549429 | 965604 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Large-scale isolation of flavan-3-ol phloroglucinol adducts by high-speed counter-current chromatography
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Large-scale isolation of flavan-3-ol phloroglucinol adducts by high-speed counter-current chromatography Large-scale isolation of flavan-3-ol phloroglucinol adducts by high-speed counter-current chromatography](/preview/png/10549429.png)
چکیده انگلیسی
Flavan-3-ol phloroglucinol adducts were synthesised through acid catalysed degradation of a procyanidins-rich grape seed extract in the presence of phloroglucinol. The reaction mixture (3.3 g) was fractionated without further sample preparation using the all-liquid chromatographic technique of high-speed counter-current chromatography (HSCCC). Selected solvent systems were hexane-ethyl acetate-methanol-water (0.1:5:0.1:5, v/v/v/v) and (1.5:10:1.5:10, v/v/v/v). The fractions obtained were found to contain almost pure compounds, in some cases final purification was achieved by preparative HPLC. The so-obtained pure standards of (+)catechin-(4αâ2)-phloroglucinol, (â)epicatechin-(4βâ2)-phloroglucinol, (+)catechine, (â)epicatechin-3-O-galloyl-(4βâ2)-phloroglucinol, (â)epicatechin, and (â)epicatechin gallate are required for quantification of acid-catalysed phloroglucinol degradation products of procyanidins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1072, Issue 2, 29 April 2005, Pages 217-222
Journal: Journal of Chromatography A - Volume 1072, Issue 2, 29 April 2005, Pages 217-222
نویسندگان
N. Köhler, P. Winterhalter,