کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10550341 | 967050 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification of IgG and insulin on supports grafted by sialic acid developing “thiophilic-like” interactions
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We developed the synthesis of new supports for the purification of insulin and IgG by affinity chromatography. The preparation of such an affinity support is performed in two steps. First, silica beads are coated with dextran polymers carrying a calculated amount of positively charged diethylaminoethyl groups in order to mask negative charges at its surface. Second, ligand is immobilized using a coupling agent. This support combines the advantages of polysaccharide phases with the excellent mechanical characteristics of silica. The existence of N-acetylneuraminic acid (sialic acid) in insulin receptor and in the antigenic determinant of IgG suggests that such an acid may develop specific interactions usable in affinity chromatography. Therefore, N-acetylneuraminic acid was used as an active ligand. The immobilization of sialic acid can be carried out by using the conventional coupling agent: the carbonyldiimidazole. The performances of these supports grafted by sialic acid were studied by high-performance liquid affinity chromatography (HPAC). The optimization of the chromatographic conditions (support characteristics and mobile phase) enabled us to observe a behavior of the type “thiophilic” of the support, which does not contain sulfone group. This new affinity support allowed a one-step separation of the IgG from mouse ascitic fluids and also allowed the insulin purification from a pancreatic extract with a good purification yields.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 818, Issue 1, 15 April 2005, Pages 53-59
Journal: Journal of Chromatography B - Volume 818, Issue 1, 15 April 2005, Pages 53-59
نویسندگان
Hamid Lakhiari, Daniel Muller,