کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10550513 | 967066 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Study on the interactions between anti-HIV-1 active compounds with trans-activation response RNA by affinity capillary electrophoresis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The study on the interactions between two anti-human immunodeficiency virus type 1 (anti-HIV-1) active compounds with trans-activation response (TAR) RNA by affinity capillary electrophoresis (ACE) with UV absorbance detection is presented. The results showed that the novel active molecules could interact with TAR RNA and inhibit the reproduce process of HIV-1. The binding constants were estimated by the change of migration time of the analytes through the change of concentrations of TAR RNA in the buffer solution. The yielded binding constants of 8.87 Ã 103 Mâ1 for active compound C3 and 8.42 Ã 103 Mâ1 for MC3 at 20.0 °C, 0.626 Ã 103 Mâ1 and 0.644 Ã 103 Mâ1 at 37.0 °C, respectively. The thermodynamic parameters ÎH and ÎS were obtained and shown that both hydrophobic and electrostatic interaction played roles in the binding processes. The results showed that the presented method was an easy and simple method to evaluate the interaction of small molecules with some bioactive materials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 814, Issue 1, 5 January 2005, Pages 99-104
Journal: Journal of Chromatography B - Volume 814, Issue 1, 5 January 2005, Pages 99-104
نویسندگان
Li Ding, Xin-Xiang Zhang, Wen-Bao Chang, Wei Lin, Ming Yang,