کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10551335 | 967117 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification
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کلمات کلیدی
Viscosity-average molecular weightATPS1-Anilino-8-naphthalene sulfonate - 1-آنیلین-8-نفتالین سولفوناتBSA - BSAmdx - MDXbovine serum albumin - آلبومین سرم گاوProtein recovery - بازیابی پروتئینTrypsin - تریپسینPartitioning - تقسیم بندیTRY - تلاش کردنANS - سالaqueous two-phase system - سیستم دوفازی آبیPartition coefficient - ضریب تقسیمlysozyme - لیزوزیم LYS - لیستMaltodextrin - مالتودکسترینProtein surface hydrophobicity - هیدروفوبیت سطح پروتئینnumber-average molecular weight - وزن مولکولی میانگین
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification](/preview/png/10551335.png)
چکیده انگلیسی
In this study, the effect of several factors that govern the partitioning behaviour of three model proteins, such as bovine serum albumin, lysozyme and trypsin was analysed in a two-phase system formed by maltodextrin and a copolymer of ethylene and propylene oxides. The protein partition coefficient (Kr) showed to be very sensitive to temperature changes, protein molecular weight, pH medium and the lyotropic ion presence. The phase diagram obtained for these novel polymer-polymer two-phase systems shows two phases with high polymer concentrations. The maltodextrin is enriched in the bottom phase while the copolymer of ethylene and propylene oxides is found in the upper phase. Since this copolymer is thermoreactive, the upper phase can be removed and heated above the copolymer's cloud point resulting in the formation of a new two-phase system with a lower water phase, containing the target protein and an upper copolymer-rich phase. Our results show that systems formed by maltodextrin and a copolymer of ethylene and propylene oxides may be considered as an interesting alternative to be used in protein purification due to their low cost, and also because they offer a viable solution to problems of polymer removal and recycling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 814, Issue 2, 25 January 2005, Pages 347-353
Journal: Journal of Chromatography B - Volume 814, Issue 2, 25 January 2005, Pages 347-353
نویسندگان
Belén Bolognese, Bibiana Nerli, Guillermo Picó,