کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10552264 | 967197 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Liquid chromatography-tandem mass spectrometry determination of LSD, ISO-LSD, and the main metabolite 2-oxo-3-hydroxy-LSD in forensic samples and application in a forensic case
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کلمات کلیدی
ESIR.S.D.2-oxo-3-hydroxy-LSDRIAMRMLSDLOQGC/MS - GC / MSLC/MS/MS - LC / MS / MSinternal standard - استاندارد داخلیLysergic acid diethylamide - اسید لیزرژیک دی اتیل آمیدrelative standard deviation - انحراف استاندارد نسبیNumber of samples - تعداد نمونه هاWhole blood - خون کاملradioimmunoassay - رادیوایمونواسیBias - سوگیری یا سوگراییCorrelation coefficient - ضریب همبستگیlimit of quantification - محدودیت اندازه گیریmultiple reaction monitoring - نظارت چندگانه چندگانه
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A liquid chromatography mass spectrometric (LC/MS/MS) method has been developed for the determination of LSD, iso-LSD and the metabolite 2-oxo-3-hydroxy-LSD in forensic applications. The procedure involves liquid-liquid extraction of the analytes and LSD-D3 (internal standard) from 1.0 g whole blood or 1.0 ml urine with butyl acetate at pH 9.8. Confirmation and quantification were done by positive electrospray ionisation with a triple quadrupole mass spectrometer operating in multiple reaction monitoring (MRM) mode. Two MRM transitions of each compound were established and identification criteria were set up based on the retention time and the ion ratio. The curves of extracted standards were linear over a working range of 0.01-50 μg/kg for all transitions of LSD and iso-LSD. The limit of quantification was 0.01 μg/kg for LSD and iso-LSD. The method was applied to a case investigation involving a 26-year-old male suspected for having attempted homicide, where blood concentrations of LSD and iso-LSD were determined to 0.27 and 0.44 μg/kg, respectively. 2-Oxo-3-hydroxy-LSD was detected in the urine and confirmed the LSD abuse. The case illustrated the importance of analyte separation before MRM detection of a sample due to identical fragmentation ions of the isomers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 825, Issue 1, 15 October 2005, Pages 21-28
Journal: Journal of Chromatography B - Volume 825, Issue 1, 15 October 2005, Pages 21-28
نویسندگان
Sys Stybe Johansen, Jytte Lundsby Jensen,