Method development for β-glucogallin and gallic acid analysis: Application to urinary pharmacokinetic studies

A reversed phase high-pressure liquid chromatographic electrospray tandem mass spectrometry method was developed for the simultaneous detection and analysis of β-glucogallin and gallic acid. This method used a C18 column with ultraviolet detection at 285 nm, ionization in the negative ion mode for β-glucogallin and gallic acid, and in the positive ion mode for the internal standard 3-morpholin-4-ylpropane-1-sulfonic acid. Mobile phase consisted of a mixture of water, methanol, and formic acid at a flow rate of 0.7 mL/min. This method was validated over a concentration range of 1-100 μg/mL in rat urine.