The enzyme-linked immunosorbent assay (ELISA) method for nicotine metabolites determination in biological fluids

The ELISA method, used in determination of the main nicotine metabolites showed high accuracy and sensitivity. However, this method was less specific than the reference technique (TLC). The high correlation coefficients, r > 0.9, between the results of determined nicotine metabolites in urine by means of ELISA and TLC with densitometry confirmed the possibility of the application of ELISA method to practical monitoring of tobacco smoke exposure in large population groups.