کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10553706 | 967909 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A stability-indicating HPLC assay for metronidazole benzoate
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A stability-indicating HPLC assay for metronidazole benzoate A stability-indicating HPLC assay for metronidazole benzoate](/preview/png/10553706.png)
چکیده انگلیسی
A simple and rapid stability-indicating HPLC assay procedure has been developed and validated for metronidazole benzoate. The HPLC conditions were as follows, column: Waters Symmetry C8, 5 μm packing, 4.6 mm Ã 250 mm; detection: UV at 271 nm; injection volume: 20 μl; mobile phase: acetonitrile-0.1% glacial acetic acid in monobasic potassium phosphate (0.01 M) (40:60, v/v); isocratic elution under ambient temperature at 2.0 ml minâ1. The procedure separated metronidazole benzoate and its potential degradation products, metronidazole and benzoic acid, in an overall analysis time of about 6 min with metronidazole benzoate eluting at about 5 min. The injection repeatability was 0.03%, and the intraday and interday repeatability were 0.4 and 0.7%, respectively. The procedure provided a linear response over the concentration range 0.2-800 μg mlâ1 (r = 1.0000) with the limits of detection and quantitation 0.03 and 0.2 μg mlâ1, respectively. The solubilities of metronidazole benzoate in water, 0.01 M hydrochloric acid and 0.05 M phosphate buffer, pH 6.8, determined each in triplicate using the procedure, were 0.2 mg mlâ1 (R.S.D. 7%), 0.4 mg mlâ1 (R.S.D. 2%) and 0.2 mg mlâ1 (R.S.D. 8%), respectively. The results show no detectable hydrolysis of metronidazole benzoate in 0.01 M hydrochloric acid at 37 °C or in the mobile phase at ambient temperature in 10 h.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 4, 15 July 2005, Pages 776-780
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 4, 15 July 2005, Pages 776-780
نویسندگان
Daniel K. Bempong, Ronald G. Manning, Tahseen Mirza, Lokesh Bhattacharyya,