کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10554340 | 967959 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method](/preview/png/10554340.png)
چکیده انگلیسی
A simple, sensitive and specific high-performance liquid chromatography-UV (HPLC-UV) method has been developed for the first time to simultaneously quantify the six major active saponins of Panax notoginseng, namely notoginsenoside R1, ginsenoside Rg1, Rb1, Rg2, Rh1 and Rd. Astragaloside IV is used as the internal standard. This HPLC assay was performed on a reversed-phase C18 column with gradient elution of acetonitrile and 0.01% formic acid in 30Â min. The method provided good reproducibility and sensitivity for the quantification of six saponins with overall intra- and inter-day precision and accuracy of less than 4.0% and higher than 90%, respectively. This assay is successfully applied to the determination of the six saponins in 23 notoginseng samples. The results indicated that the developed HPLC assay can be readily utilized as a quality control method for P. notoginseng.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 1, 1 June 2005, Pages 45-51
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 38, Issue 1, 1 June 2005, Pages 45-51
نویسندگان
Lie Li, Jin-lan Zhang, Yu-xin Sheng, De-an Guo, Qiao Wang, Hong-zhu Guo,