کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10554466 967963 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High-performance liquid chromatographic determination of enalapril in human plasma by enzyme kinetic analytical method
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
High-performance liquid chromatographic determination of enalapril in human plasma by enzyme kinetic analytical method
چکیده انگلیسی
A high-performance liquid chromatographic method for indirect determination of enalapril in human plasma, was developed and validated. An exogenous angiotensin converting enzyme after drug inhibition was determined by reacting with hippuryl-histidyl-leucine to produce hippuric acid (HA) which was inversely proportional to the amount of enalaprilat in plasma. The HPLC was carried out on a Lichrosphere® 60RP-select B, C18, 5 μm (125 mm × 4.0 mm i.d.) column at flow rate of 1.0 ml/min. The analysis time per injection was within 6.5 min. The lowest concentration of enalaprilat to be quantitated was 3.0 ng/ml with the acceptable accuracy and precision. This successfully developed method was practically and accurately used for pharmacokinetics and bioequivalent study of enalapril.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 37, Issue 4, 1 April 2005, Pages 763-769
نویسندگان
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