کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10556129 | 968221 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deconvolution of overlapping isotopic clusters improves quantification of stable isotope-labeled peptides
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
⺠When the mass shift between the labeled peptides is small, there can be the possibility for overlap of the isotopic clusters which will hamper quantification accuracy with a typical upwards bias for the heavier peptide. ⺠We investigated the impact of the overlapping peak issue with respect to dimethyl based quantification and we confirmed the need for correction. ⺠We present a tool that can correct overlapping issues when they arise which is based on modeling isotopic distributions. ⺠We demonstrate that our approach leads to improved accuracy and precision of protein quantification.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 74, Issue 10, 6 September 2011, Pages 2204-2209
Journal: Journal of Proteomics - Volume 74, Issue 10, 6 September 2011, Pages 2204-2209
نویسندگان
Salvatore Cappadona, Javier Muñoz, Wim P.E. Spee, Teck Y. Low, Shabaz Mohammed, Bas van Breukelen, Albert J.R. Heck,